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【现货】L-929细胞mDdx3x因敲除株(CGKO-M2127)
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类型
基因敲除
品牌
Cas9x
产品货号
CGKO-M2127
价格
10000.00
库存量
100
用途
仅供科研使用

产品名称:L-929细胞mDdx3x因敲除株

产品简称L-929 KO-mDdx3x

产品货号CGKO-M2127

修饰基因:mDdx3x

因ID13205

修饰类型:敲除

转导方法:质粒电转

抗性基因:Puro

克隆类型:纯合子

细胞鉴定:PCR,测序

种属来源小鼠

组织来源皮下结缔组织

疾病特征:/

细胞形态成纤维细胞样

生长特性贴壁生长

培养体系MEM+10%马血清+1%P/S

配套培养基货号:TCM-G749

传代比例:1:3-1:6,每2-3天换液一次

传代周期48-72 h

培养条件气相:95%空气+5%CO2,温度:37℃

冻存条件60%基础培养基+30%FBS+10%DMSO,液氮储存

质量检测细菌、真菌、支原体检测均为阴性

参考文献:

"Kazazian HH Jr., et al. Restriction site polymorphism in the phosphoglycerate kinase gene on the X chromosome. Hum. Genet. 66: 217-219, 1984. PubMed: 6325324

Fisher EM, et al. Homologous ribosomal protein genes on the human X and Y chromosomes: escape from X inactivation and possible implications for Turner syndrome. Cell 63: 1205-1218, 1990. PubMed: 2124517

Sanford KK, et al. The growth in vitro of single isolated tissue cells. J. Natl. Cancer Inst. 9: 229-246, 1948.

Sugarman BJ, et al. Recombinant human tumor necrosis factor-alpha: effects on proliferation of normal and transformed cells in vitro. Science 230: 943-945, 1985. PubMed: 393311

ASTM International Standard Practice for Direct Contact Cell Culture Evaluation of Materials for Medical Devices. West Conshohocken, PA:ASTM International;ASTM Standard Test Method F 0813-07.

ASTM International Standard Test Method for Agar Diffusion Cell Culture Screening for Cytotoxicity. West Conshohocken, PA:ASTM International;ASTM Standard Test Method F 0895-84 (Reapproved 2006).

U.S. Pharmacopeia General Chapters:<87>Biological Reactivity Tests, in vitro. Rockville, MD: U.S. Pharmacopeia; USP USP34-NF29, 2011

Westfall BB, et al. The glycogen content of cell suspensions prepared from massive tissue culture: comparison of cells derived from mouse connective tissue and mouse liver. J. Natl. Cancer Inst. 14: 655-664, 1953. PubMed: 13233820

Earle WR, et al. Production of malignancy in vitro. IV. The mouse fibroblast cultures and changes seen in the living cells. J. Natl. Cancer Inst. 4: 165-212, 1943."


收货当天如何处理细胞?

收到细胞后请务必仔细阅读产品资料,了解细胞相关信息,如贴壁特性 (贴壁/悬浮/半贴壁半悬浮)、细胞形态、培养体系、传代比例和换液频率等。

点击可查看下载《细胞收货处理方法说明》.jpg

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