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产品名称：Lncap细胞hFOXA1基因敲除株

产品简称：Lncap KO-hFOXA1

产品货号：CGKO-M0012

修饰基因：hFOXA1

基因ID：3169

修饰类型：敲除

转导方法：蛋白电转

抗性基因：—

克隆类型：纯合子

细胞鉴定：PCR，测序

种属来源：人

组织来源：前列腺

疾病特征：前列腺癌

细胞形态：上皮细胞样

生长特性：贴壁生长

培养体系：RPMI-1640+10%FBS+1%P/S

配套培养基货号：TCH-G238

传代比例：1:2-1:3，每2-3天换液一次

传代周期：72-96 h

培养条件：气相：95%空气+5%CO2，温度：37℃

冻存条件：60%基础培养基+30%FBS+10%DMSO，液氮储存

质量检测：细菌、真菌、支原体检测均为阴性

参考文献：

Models for prostate cancer. 37New York: Liss; 1980.

Gibas Z, et al. A high-resolution study of chromosome changes in a human prostatic carcinoma cell line (LNCaP). Cancer Genet. Cytogenet. 11: 399-404, 1984. PubMed: 6584201

Horoszewicz JS, et al. LNCaP model of human prostatic carcinoma. Cancer Res. 43: 1809-1818, 1983. PubMed: 6831420

Hu SX, et al. Development of an adenovirus vector with tetracycline-regulatable human tumor necrosis factor alpha gene expression. Cancer Res. 57: 3339-3343, 1997. PubMed: 9269991

Boffa LC, et al. Invasion of the CAG triplet repeats by a complementary peptide nucleic acid inhibits transcription of the androgen receptor and TATA-binding protein genes and correlates with refolding of an active nucleosome containing a unique AR gene sequence. J. Biol. Chem. 271: 13228-13233, 1996. PubMed: 8662737

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