8000.00
【现货】DLD-1细胞hBRCA1基因敲除株(CGKO-M2435)
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类型
基因敲除
品牌
Cas9x
产品货号
CGKO-M2435
价格
8000.00
库存量
100
用途
仅供科研使用

产品名称:DLD-1细胞hBRCA1基因敲除株

产品简称DLD-1 KO-hBRCA1

产品货号CGKO-M2435

修饰基因:hBRCA1

因ID 672

修饰类型:敲除

转导方法:蛋白电转

抗性基因:

克隆类型:杂合子

细胞鉴定:PCR,测序

种属来源

组织来源结直肠

疾病特征结直肠腺癌

细胞形态上皮细胞样

生长特性贴壁生长

培养体系RPMI-1640+10%FBS+1%P/S

配套培养基货号:TCH-G169

传代比例:1:3-1:4,每2-3天换液一次

传代周期48-72 h

培养条件气相:95%空气+5%CO2,温度:37℃

冻存条件60%基础培养基+30%FBS+10%DMSO,液氮储存

质量检测细菌、真菌、支原体检测均为阴性

参考文献:

Chen TR, et al. Intercellular karyotypic similarity in near-diploid cell lines of human tumor origins. Cancer Genet. Cytogenet. 10: 351-362, 1983. PubMed: 6652615

Chen TR, et al. DLD-1 and HCT-15 cell lines derived separately from colorectal carcinomas have totally different chromosome changes but the same genetic origin. Cancer Genet. Cytogenet. 81: 103-108, 1995. PubMed: 7621404

Trainer DL, et al. Biological characterization and oncogene expression in human colorectal carcinoma cell lines. Int. J. Cancer 41: 287-296, 1988. PubMed: 3338874

Dexter DL, et al. N,N-dimethylformamide-induced alteration of cell culture characteristics and loss of tumorigenicity in cultured human colon carcinoma cells. Cancer Res. 39: 1020-1025, 1979. PubMed: 427742

Rodrigues NR, et al. p53 mutations in colorectal cancer. Proc. Natl. Acad. Sci. USA 87: 7555-7559, 1990. PubMed: 1699228

Keesee SK, et al. Nuclear matrix proteins in human colon cancer. Proc. Natl. Acad. Sci. USA 91: 1913-1916, 1994. PubMed: 8127905

Kutchera W, et al. Protaglandin H synthase 2 is expressed abnormally in human colon cancer: evidence for a transcriptional effect. Proc. Natl. Acad. Sci. USA 93: 4816-4820, 1996. PubMed: 8643486

Vermeulen SJ, et al. Did the four human cancer cell lines DLD-1, HCT-15, HCT-8, and HRT-18 originate from one and the same patient?. Cancer Genet. Cytogenet. 107: 76-79, 1998. PubMed: 9809040


收货当天如何处理细胞?

收到细胞后请务必仔细阅读产品资料,了解细胞相关信息,如贴壁特性 (贴壁/悬浮/半贴壁半悬浮)、细胞形态、培养体系、传代比例和换液频率等。

点击可查看下载《细胞收货处理方法说明》.jpg

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