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G.E. Foley 等人建立了类淋巴母细胞细胞株CCRF-CEM。 细胞是1964年11月从一位四岁白人女性急性淋巴细胞白血病患者的外周血白血球衣中得到。所供 Luciferase 稳转细胞株采用VirusFree系统构建，除稳定高效地表达 luciferase 基因外，还具有特异性强、成像质量高、发光强度可精确定量等优点，可实现包括启动子活性研究、哺乳动物细胞双杂交实验以及活体动物成像实验等多方面的灵活应用。
基因报告系统广泛应用于真核生物基因表达和细胞生理学的研究，是提高实验准确度的常用方法。荧光素酶（Luciferase）是以荧光素（luciferin）或脂肪醛（firefly aldehyde）为底物来检测荧光素酶活性的一种常见基因报告系统，因其具有方便快捷、灵敏度强、成功率高等优点，在基因表达的研究中得到广泛应用。
细胞名称：荧光素酶标记的人急性淋巴细胞白血病T淋巴细胞
细胞简称：CCRF-CEM-Luc
产品货号：TCH-C155L
转染方法：VirusFree 系统
抗性：Puro
靶细胞名称：CCRF-CEM
靶细胞别名：CCRF/CEM; CCRFCEM; CCRF.CEM; CCRF CEM; CCRF; CEM; CEM-CCRF; CEM-CCRF (CAMR); CCRF/CEM/0; CEM/0; CEM-0; CCRF-CEM/S; GM03671; GM03671C
种属来源：人
组织来源：外周血
疾病特征：急性淋巴细胞白血病
细胞形态：淋巴母细胞样
生长特性：悬浮生长
培养体系：RPMI-1640＋10% FBS+0.5 ug/mL Puromycin＋1% P/S
配套培养基货号：TCH-G155L
传代比例：3×10^5-5×10^5cells/mL，每2-3天换液一次
倍增时间：24-48 h
培养条件：气相：95%空气+5%CO2，温度：37℃
冻存条件：60%基础培养基+30%FBS+10%DMSO，液氮储存
质量检测：细菌、真菌、支原体检测均为阴性
参考文献：
Foley GE et al. Continous culture of human lymphoblasts from peripheral blood of a child with acute leukemia. Cancer 18: 522-529, 1965 Sandstrom PA and Buttke TM. Autocrine production of extracellular catalase prevents apoptosis of the human CEM T-cell line in serum-free medium. Proc. Natl Acad. Sci. USA 90:4708-4712, 1993 PubMed: 93281648 Miranda L et al. Isolation of the human PC6 gene encoding the putative host protease for HIV-1 gpl60 processing in CD4+ T lymphocytes. Proc. Natl. Acad. Sci. USA 93: 7695-7700, 1996 PubMed: 96353880 Adams RA. Formal discussion: the role of transplantation in the experimental investigation of human leukemia and lymphoma. Cancer Res. 27: 2479-2482, 1967 PubMed: 68134846 Uzman BG et ah Morphologic variations in human leukemic lymphoblasts (CCRF-CEM cells) after long-term culture and exposure to chemotherapeutic agents. A study with the electron microscope. Cancer 19:1725-I742, 1966 PubMed: 67(146473 Adams RA et al. Leukemia: serial transplantation of human leukemic lymphoblasts in the newborn Syrian halnster. Cancer Res. 27: 772-783. 1967 PubMed: 68122005 Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2~a edition, Published by ATCC. Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1 q88. Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D.,(1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC. Centers for Disease Control (1993), Biosafety in Microbiological and Biomedical Laboratories Human Health Service Publication No. (CDC) 93-8395. U.S Dept. of Health and Human Services; 3rd Edition U.S. Government Printing Office Washington D.C.
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