HyCyte® | 海星生物

发货周期：

干冰冻存管运输3天（1x10⁶cells/冻存管x2）

常温运温1-2周发货 (1x10⁶ cells/T25培养瓶）

原价：1800元   套装价特惠价：890元

一名低分化的胚胎神经母细胞瘤患儿的骨髓转移

细胞名称：人神经母细胞瘤细胞（STR鉴定正确）

细胞简称：SK-N-AS

产品货号：TCH-C378

种属来源：人

组织来源：脑

疾病特征：神经母细胞瘤

细胞形态：上皮细胞样

生长特性：贴壁生长

培养体系：DMEM-H+10%FBS+1% NEAA+1%P/S

配套培养基货号：TCH-G378

传代比例：1:2-1:3，每2-3天换液一次

传代周期：72-96 h

培养条件：气相：95%空气+5%CO2，温度：37℃

冻存条件：60%基础培养基+30%FBS+10%DMSO，液氮储存

质量检测：细菌、真菌、支原体检测均为阴性

参考文献：

Sugimoto T, et al. Determination of cell surface membrane antigens common to both human neuroblastoma and leukemia-lymphoma cell lines by a panel of 38 monoclonal antibodies. J. Natl. Cancer Inst. 73: 51-57, 1984. PubMed: 6610792

Iavarone A, et al. Uptake and storage of m-iodobenzylguanidine are frequent neuronal functions of human neuroblastoma cell lines. Cancer Res. 53: 304-309, 1993. PubMed: 8417824

Gaetano C, et al. Retinoic acid negatively regulates p34cdc2 expression during human neuroblastoma differentiation. Cell Growth Differ. 2: 487-493, 1991. PubMed: 1751405

El-Badry OM, et al. Autonomous growth of a human neuroblastoma cell line is mediated by insulin-like growth factor II. J. Clin. Invest. 84: 829-839, 1989. PubMed: 2547840

bone marrow metastasis from a child with poorly differentiated embryonal neuroblastoma

SK-N-AS 已发表过的文章：

IF=3.6 Mengzhen Z, Xinwei H, Zeheng T, et al. Integrated machine learning-driven disulfidptosis profiling: CYFIP1 and EMILIN1 as therapeutic nodes in neuroblastoma[J]. Journal of Cancer Research and Clinical Oncology, 2024, 150(3): 109.

收货当天如何处理细胞？

收到细胞后请务必仔细阅读产品资料，了解细胞相关信息，如贴壁特性 (贴壁/悬浮/半贴壁半悬浮)、细胞形态、培养体系、传代比例和换液频率等。