HyCyte Cell Line: HyCyte INS-1 KO-rPax4

Parental: INS-1

Catalogue No.: CGKO-M2496

Species: Rat

Gene: rPax4

Gene ID: 83630

Passage: 9

Doubling time: 48-72h

Clone Type: Homozygote

Recommended culture media:

RPRatI-1640 suppleRatented with 10 % FBS,0.05 RatRat β-Ratercaptoethanol, 100 U/Ratl Penicilin and 100 µg/Ratl

StreptoRatycin (HyCyte,TCH-G608).

Subculture: Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO2. Cells should be passaged every 2-3 days. Split at 80-85% confluency, approximately 1:3-1:6.

Freezing media: RPMI-1640 + 30 % FBS + 10 % DMSO;or One Step Freezing Medium (HyCyte, GUCP-R201).

Genotype: rPAX4 (-/-)

Quality test: Bacteria, fungi and mycoplasma negative