HyCyte Cell Line: HyCyte NCI-H295R KO-hNPC1

Parental: NCI-H295R

Catalogue No.: CGKO-M2615

Species: Human

Gene: hNPC1

Gene ID: 4864

Passage: 9

Doubling time: 36-48h

Growth Properties: Adherent

Clone Type: Homozygote

Recommended culture media:

Dulbecco's Modified Eagle Medium/Nutrient Mixture F-12 (D/F12) supplemented with 10 % FBS, 1% Insulin-Transferrin-Selenium Supplement, 100 U/ml Penicilin and 100 µg/ml Streptomycin (HyCyte,TCH-G578).

Subculture: Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO₂. Cells should be passaged every 2-3 days. Split at 80-85% confluency, approximately 1:2-1:4.

Freezing media: D/F12 + 30 % FBS + 10 % DMSO;or One Step Freezing Medium (HyCyte, GUCP-R201).

Genotype: hNPC1 (-/-)

Quality test: Bacteria, fungi and mycoplasma negative