HyCyte Cell Line: HyCyte AML12 KO-mRtn4

Parental: AML12

Catalogue No.: CGKO-M2718

Species: Mouse

Gene: mRtn4

Gene ID: 68585

Passage: 9

Doubling time: 24-48h

Growth Properties: Adherent

Clone Type: Homozygote

Recommended culture media:

Dulbecco's Modified Eagle Medium/Nutrient Mixture F-12 (D/F12) supplemented with 10 % FBS,40ng/mL Dexamethasone, 100 U/ml Penicilin and 100 µg/ml

Streptomycin (HyCyte,TCM-G709).

Subculture: Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO₂. Cells should be passaged every 2-3 days. Split at 80-85% confluency, approximately 1:2-1:4.

Freezing media: RPMI-1640 + 30 % FBS + 10 % DMSO;or One Step Freezing Medium(HyCyte, GUCP-R201).

Genotype: mRtn4 (-/-)

Quality test: Bacteria, fungi and mycoplasma negative