HyCyte Cell Line: HyCyte MIN6 KO-mSec61a2

Parental: MIN6

Catalogue No.: CGKO-M2828

Species: Mouse

Gene: mSec61a2

Gene ID: 57743

Passage: 9

Doubling time: 72-96 h

Growth Properties: Adherent

Clone Type: Homozygote

Recommended culture media:

Dulbecco's Modified Eagle Medium ,High Glucose(DMEM) supplemented with 15 % FBS, 2mM Glutamax, 0.05 mM β-mercaptoethanol, 100 U/mL Penicilin and 100 µg/mL Streptomycin (HyCyte,TCM-G787).

Subculture: Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO₂. Cells should be passaged every 2-3 days. Split at 80-85% confluency, approximately 1:2-1:3.

Freezing media: DMEM + 30 % FBS + 10 % DMSO;or One Step Freezing Medium (HyCyte, GUCP-R201).

Genotype: mSec61a2 (-/-)

Quality test: Bacteria, fungi and mycoplasma negative