HyCyte Cell Line: HyCyte RenCa-Luc KO-mPbrm1

Parental: RenCa-Luc

Catalogue No.: CGKO-M2887

Species: Mouse

Gene: mPbrm1

Gene ID: 66923

Passage: 9

Doubling time: 48-72 h

Growth Properties: Adherent

Clone Type: Homozygote

Recommended culture media:

RPMI-1640 supplemented with 10 % FBS, 2mM Glutamax,1mM Sodium Pyruvate,1% NEAA, 2.0 ug/mL Puromycin, 100 U/ml Penicilin and 100 µg/ml Streptomycin (HyCyte,TCM-G784).

Subculture: Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO₂. Cells should be passaged every 2-3 days. Split at 80-85% confluency, approximately 1:3-1:5.

Freezing media: RPMI-1640 + 30 % FBS + 10 % DMSO;or One Step Freezing Medium(HyCyte, GUCP-R201).

Genotype: mPbrm1 (-/-)

Quality test: Bacteria, fungi and mycoplasma negative