HyCyte Cell Line: HyCyte MH-S KO-mSirt2

Parental: MH-S

Catalogue No.: CGKO-M2889

Species: Mouse

Gene: mSirt2

Gene ID: 64383

Passage: 9

Doubling time: 24-48 h

Growth Properties: Semi-adherent

Clone Type: Homozygote

Recommended culture media:

RPMI-1640 supplemented with 10 % FBS,0.05 mM β-mercaptoethanol, 100 U/ml Penicilin and 100 µg/ml Streptomycin (HyCyte,TCM-G795).

Subculture: Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO₂. Cells should be passaged every 2-3 days. Split at 80-85% confluency, approximately 1:2-1:4.

Freezing media: RPMI-1640 + 30 % FBS + 10 % DMSO;or One Step Freezing Medium(HyCyte, GUCP-R201).

Genotype: mSirt2 (-/-)

Quality test: Bacteria, fungi and mycoplasma negative