HyCyte Cell Line: HyCyte H9c2(2-1) KO-rLOC102549962

Parental: H9c2(2-1)

Catalogue No.: CGKO-M2932

Species: Rat

Gene: rLOC102549962

Gene ID: 102549962

Passage: 9

Doubling time: 48-72 h

Growth Properties: Adherent

Clone Type: Homozygote

Recommended culture media:

Dulbecco's Modified Eagle Medium ,High Glucose(DMEM) supplemented with 10 % FBS, 2mM Glutamax,100 U/mL Penicilin and 100 µg/mL Streptomycin (HyCyte,TCR-G607).

Subculture: Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO₂. Cells should be passaged every 2-3 days. Split at 80-85% confluency, approximately 1:3-1:4.

Freezing media: DMEM + 30 % FBS + 10 % DMSO;or One Step Freezing Medium (HyCyte, GUCP-R201).

Genotype: rLOC102549962 (-/-)

Quality test: Bacteria, fungi and mycoplasma negative