HyCyte Cell Line: HyCyte WPMY-1 KO-hHIF1A

Parental: WPMY-1

Catalogue No.: CGKO-M2934

Species: Human

Gene: hHIF1A

Gene ID: 3091

Passage: 9

Doubling time: 24-48 h

Growth Properties: Adherent

Clone Type: Homozygote

Recommended culture media:

Dulbecco's Modified Eagle Medium ,High Glucose(DMEM) supplemented with 5 % FBS, 100 U/mL Penicilin and 100 µg/mL Streptomycin (HyCyte,TCH-G374).

Subculture: Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO₂. Cells should be passaged every 2-3 days. Split at 80-85% confluency, approximately 1:2-1:4.

Freezing media: DMEM + 30 % FBS + 10 % DMSO;or One Step Freezing Medium (HyCyte, GUCP-R201).

Genotype: hHIF1A (-/-)

Quality test: Bacteria, fungi and mycoplasma negative