HyCyte Cell Line: HyCyte B16-F10 KO-mPrmt3

Parental: B16-F10

Catalogue No.: CGKO-M2953

Species: Mouse

Gene: mPrmt3

Gene ID: 71974

Passage: 9

Doubling time: 48-72 h

Growth Properties: Adherent

Clone Type: Homozygote

Recommended culture media:

Dulbecco's Modified Eagle Medium ,High Glucose(DMEM) supplemented with 10 % FBS, 100 U/ml Penicilin and 100 µg/ml

Streptomycin (HyCyte,TCM-G713).

Subculture: Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO₂. Cells should be passaged every 2-3 days. Split at 80-85% confluency, approximately 1:3-1:6.

Freezing media: DMEM + 30 % FBS + 10 % DMSO;or One Step Freezing Medium (HyCyte, GUCP-R201).

Genotype: mPrmt3 (-/-)

Quality test: Bacteria, fungi and mycoplasma negative