HyCyte Cell Line: HyCyte 3T3-L1 KO-mSfrp5

Parental: 3T3-L1

Catalogue No.: CGKO-M2978

Species: Mouse

Gene: mSfrp5

Gene ID: 54612

Passage: 9

Doubling time: 48-72 h

Growth Properties: Adherent

Clone Type: Homozygote

Recommended culture media:

Dulbecco's Modified Eagle Medium ,High Glucose(DMEM) supplemented with 10 % FBS, 100 U/ml Penicilin and 100 µg/ml

Streptomycin (HyCyte,TCM-G702).

Subculture: Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO₂. Cells should be passaged every 2-3 days. Split at 80-85% confluency, approximately 1:3-1:4.

Freezing media: DMEM + 30 % FBS + 10 % DMSO;or One Step Freezing Medium (HyCyte, GUCP-R201).

Genotype: mSfrp5 (-/-)

Quality test: Bacteria, fungi and mycoplasma negative