HyCyte Cell Line: HyCyte Ishikawa KO-hCTNNBIP1

Parental: Ishikawa

Catalogue No.: CGKO-M3000

Species: Human

Gene: hCTNNBIP1

Gene ID: 56998

Passage: 9

Doubling time: 24-48 h

Growth Properties: Adherent

Clone Type: Homozygote

Recommended culture media:

Minimum Essential Medium (MEM) supplemented with 15 % FBS, 2mM Glutamax,1% NEAA,100 U/ml Penicilin and 100 µg/ml Streptomycin (HyCyte,TCH-G404).

Subculture: Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO₂. Cells should be passaged every 2-3 days. Split at 80-85% confluency, approximately 1:2-1:4.

Freezing media: MEM + 30 % FBS + 10 % DMSO;or One Step Freezing Medium (HyCyte, GUCP-R201).

Genotype: hCTNNBIP1 (-/-)

Quality test: Bacteria, fungi and mycoplasma negative