HyCyte Cell Line: HyCyte PC12 KO-rYthdc2

Parental: PC12

Catalogue No.: CGKO-M3100

Species: Rat

Gene: rYthdc2

Gene ID: 307446

Passage: 9

Doubling time: 48-72 h

Growth Properties: Semi-adherent

Clone Type: Homozygote

Recommended culture media:

RPMI-1640 supplemented with 5 % FBS, 10 % HS, 100 U/ml Penicilin and 100 µg/ml Streptomycin (HyCyte,TCR-G617).

Subculture: Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO₂. Cells should be passaged every 2-3 days. Split at 80-85% confluency, approximately 1:3-1:6.

Freezing media: RPMI-1640 + 30 % FBS + 10 % DMSO;or One Step Freezing Medium(HyCyte, GUCP-R201).

Genotype: rYthdc2 (-/-)

Quality test: Bacteria, fungi and mycoplasma negative