HyCyte Cell Line: HyCyte SNU-5 KO-hSOX9

Parental: SNU-5

Catalogue No.: CGKO-M3102

Species: Human

Gene: hSOX9

Gene ID: 6662

Passage: 9

Doubling time: 36-48 h

Growth Properties: Suspension

Clone Type: Homozygote

Recommended culture media:

Iscove's Modified Dulbecco's Medium supplemented with 20 % FBS, 100 U/ml Penicilin and 100 µg/ml Streptomycin (HyCyte,TCH-G503).

Subculture: Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO₂. Cells should be passaged every 2-3 days. The cell culture density is 3×10⁵ to 5×10⁵ cells per mL.

Freezing media: IMDM + 30 % FBS + 10 % DMSO;or One Step Freezing Medium (HyCyte, GUCP-R201)

Genotype: hSOX9 (-/-)

Quality test: Bacteria, fungi and mycoplasma negative