HyCyte Cell Line: HyCyte 4T1 KO-mCd200

Parental: 4T1

Catalogue No.: CGKO-M3104

Species: Mouse

Gene: mCd200

Gene ID: 17470

Passage: 9

Doubling time: 48-72 h

Growth Properties: Adherent

Clone Type: Homozygote

Recommended culture media:

RPMI-1640 supplemented with 10 % FBS, 100 U/ml Penicilin and 100 µg/ml

Streptomycin (HyCyte,TCM-G705).

Subculture: Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO₂. Cells should be passaged every 2-3 days. Split at 80-85% confluency, approximately 1:3-1:6.

Freezing media: RPMI-1640 + 30 % FBS + 10 % DMSO;or One Step Freezing Medium(HyCyte, GUCP-R201).

Genotype: mCd200 (-/-)

Quality test: Bacteria, fungi and mycoplasma negative