HyCyte Cell Line: HyCyte RAW 264.7 KO-mSphk1

Parental: RAW 264.7

Catalogue No.: CGKO-M3194

Species: Mouse

Gene: mSphk1

Gene ID: 20698

Passage: 9

Doubling time: 48-72h

Growth Properties: Adherent

Clone Type: Homozygote

Recommended culture media:

Dulbecco's Modified Eagle Medium ,High Glucose(DMEM) supplemented with 10 % FBS, 2mM Glutamax,1mM Sodium Pyruvate100 U/ml Penicilin and 100 µg/ml

Streptomycin (HyCyte,TCM-G766).

Subculture: Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO₂. Cells should be passaged every 2-3 days. Split at 80-85% confluency, approximately 1:3-1:6.

Freezing media: DMEM + 30 % FBS + 10 % DMSO;or One Step Freezing Medium (HyCyte, GUCP-R201).

Genotype: mSphk1 (-/-)

Quality test: Bacteria, fungi and mycoplasma negative