HyCyte Cell Line: HyCyte HEK293 KO-hMTNR1A

Parental: HEK293

Catalogue No.: CGKO-M3202

Species: Human

Gene: hMTNR1A

Gene ID: 4543

Passage: 9

Doubling time: 24-48 h

Clone Type: homozygote

Recommended culture media:

Dulbecco's Modified Eagle Medium ,High Glucose(DMEM) supplemented with 10 % FBS, 100 U/mL Penicilin and 100 µg/mL Streptomycin (HyCyte,TCH-G190).

Subculture: Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO₂. Cells should be passaged every 2-3 days. Split at 80-85% confluency, approximately 1:4-1:8.

Freezing media: DMEM + 30 % FBS + 10 % DMSO; or One Step Freezing Medium (HyCyte, GUCP-R201).

Genotype: hMTNR1A (-/-)

Quality test: Bacteria, fungi and mycoplasma negative