HyCyte Cell Line: HyCyte TM4 KO-mGja1

Parental: TM4

Catalogue No.: CGKO-M3234

Species: Mouse

Gene: mGja1

Gene ID: 14609

Passage: 9

Doubling time: 18-24 h

Growth Properties: Adherent

Clone Type: Homozygote

Recommended culture media:

Dulbecco's Modified Eagle Medium/Nutrient Mixture F-12 (D/F12) supplemented with 2.5 % FBS, 5 % HS, 100 U/ml Penicilin and 100 µg/ml Streptomycin (HyCyte,TCM-G828).

Subculture: Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO₂. Cells should be passaged every 2-3 days. Split at 80-85% confluency, approximately 1:3-1:4.

Freezing media: D/F12 + 30 % FBS + 10 % DMSO;or One Step Freezing Medium (HyCyte, GUCP-R201).

Genotype: mGja1 (-/-)

Quality test: Bacteria, fungi and mycoplasma negative