HyCyte Cell Line: HyCyte SaOS-2 KO-hRBFOX1

Parental: SaOS-2

Catalogue No.: CGKO-M3270

Species: Human

Gene: hRBFOX1

Gene ID: 54715

Passage: 9

Doubling time: 72-96h

Growth Properties: Suspension

Clone Type: Homozygote

Recommended culture media:

McCoy's 5A Medium supplemented with 15 % FBS, 100 U/ml Penicilin and 100 µg/ml Streptomycin (HyCyte,TCH-G315).

Subculture: Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO2. Cells should be passaged every 2-3 days. Split at 80-85% confluency, approximately 1:3-1:6.

Freezing media: McCoy's 5A + 30 % FBS + 10 % DMSO;or One Step Freezing Medium (HyCyte, GUCP-R201)

Genotype: hRBFOX1 (-/-)

Quality test: Bacteria, fungi and mycoplasma negative