HyCyte Cell Line: HyCyte NK-92MI KO-hGZMB

Parental: NK-92MI

Catalogue No.: CGKO-M3281

Species: Human

Gene: hGZMB

Gene ID: 3002

Passage: 9

Doubling time: 48-72 h

Growth Properties: Adherent

Clone Type: Homozygote

Recommended culture media:

Minimum Essential Medium α(MEM α)supplemented with 12.5 % FBS, 12.5 % HS, 0.2mM Inositol, 0.05 mM β-mercaptoethanol, 0.02mM Folic Acid, 100 U/mL Penicilin and 100 µg/mL Streptomycin (HyCyte,TCH-G293).

Subculture: Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO2. Cells should be passaged every 2-3 days. The cell culture density is 5×10⁵ to 1×10⁶ cells per mL.

Freezing media:MEMα + 30 % FBS + 10 % DMSO;or One Step Freezing Medium (HyCyte, GUCP-R201)

Genotype: hGZMB (-/-)

Quality test: Bacteria, fungi and mycoplasma negative