HyCyte Cell Line: HyCyte NCM460 KO-hMAP3K20

Parental: NCM460

Catalogue No.: CGKO-M3296

Species: Human

Gene: hMAP3K20

Gene ID: 51776

Passage: 9

Doubling time: 18-24h

Growth Properties: Adherent

Clone Type: Homozygote

Recommended culture media:

RPMI-1640 supplemented with 10 % FBS, 100 U/ml Penicilin and 100 µg/ml Streptomycin (HyCyte,TCH-G450).

Subculture:Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO2. Cells should be passaged every 2-3 days. Split at 80-85% confluency, approximately 1:2-1:6.

Freezing media:RPMI-1640 + 30 % FBS + 10 % DMSO;or One Step Freezing Medium(HyCyte, GUCP-R201).

Genotype: hMAP3K20 (-/-)

Quality test: Bacteria, fungi and mycoplasma negative