HyCyte Cell Line: HyCyte SW1990 KO-hPHGDH

Parental: SW1990

Catalogue No.: CGKO-M3338

Species: Human

Gene: hPHGDH

Gene ID: 26227

Passage: 9

Doubling time: 48-60h

Growth Properties：Adherent

Clone Type: Homozygote

Recommended culture media:

Leibovitz L-15 Medium supplemented with 10 % FBS, 100 U/ml Penicilin and 100 µg/ml

Streptomycin (HyCyte,TCH-G343).

Subculture: Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO₂. Cells should be passaged every 2-3 days. Split at 80-85% confluency, approximately 1:2-1:4.

Freezing media: L-15+ 30 % FBS + 10 % DMSO;or One Step Freezing Medium (HyCyte, GUCP-R201).

Genotype: hPHGDH (-/-)

Quality test: Bacteria, fungi and mycoplasma negative