HyCyte Cell Line: HyCyte SH-SY5Y KO-hDEGS1

Parental: SH-SY5Y

Catalogue No.: CGKO-M3376

Species: Homo sapiens, human

Gene: hDEGS1

Gene ID: 8560

Passage: 9

Doubling time: 48--60h

Growth Properties: Semi-adherent

Clone Type: Homozygote

Recommended culture media:

Dulbecco's Modified Eagle Medium/Nutrient Mixture F-12 (D/F12) supplemented with 15 % FBS, 100 U/ml Penicilin and 100 µg/ml Streptomycin (HyCyte,TCH-G325).

Subculture: Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO₂. Cells should be passaged every 2-3 days. Split at 80-85% confluency, approximately 1:3-1:4.

Freezing media: D/F12 + 30 % FBS + 10 % DMSO;or One Step Freezing Medium (HyCyte, GUCP-R201).

Genotype: hDEGS1 (-/-)

Quality test: Bacteria, fungi and mycoplasma negative