HyCyte Cell Line:HyCyte SK-OV-3 KO-hSIGLEC15

Parental: SK-OV-3

Catalogue No.: CGKO-M3381

Species: Human

Gene: hSIGLEC15

Gene ID: 284266

Passage: 9

Doubling time: 48-60h

Growth Properties: Adherent

Clone Type: Homozygote

Recommended culture media:

McCoy's 5A Medium supplemented with 10 % FBS, 100 U/ml Penicilin and 100 µg/ml Streptomycin (HyCyte,TCH-G335).

Subculture: Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO2. Cells should be passaged every 2-3 days. Split at 80-85% confluency, approximately 1:4-1:6.

Freezing media: McCoy's 5A + 30 % FBS + 10 % DMSO;or One Step Freezing Medium (HyCyte, GUCP-R201)

Genotype: hSIGLEC15 (-/-)

Quality Control:
Negative for bacteria, fungi, and mycoplasma detection