HyCyte Cell Line:HyCyte PC-9 KO-hTP53

Parental: PC-9

Catalogue No.: CGKO-M3394

Species: Human

Gene: hTP53

Gene ID: 7157

Passage: 9

Doubling time: 24-32h

Growth Properties: Adherent

Clone Type: Homozygote

Recommended culture media:

RPMI-1640 supplemented with 10 % FBS, 2mM Glutamax,1mM Sodium Pyruvate,100 U/mL Penicilin and 100 µg/mL Streptomycin (HyCyte,TCH-G380).

Subculture: Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO2. Cells should be passaged every 2-3 days. Split at 80-85% confluency, approximately 1:4-1:6.

Freezing media: RPMI-1640 + 30 % FBS + 10 % DMSO;or One Step Freezing Medium(HyCyte, GUCP-R201).

Genotype: hTP53 (-/-)

Quality test: Negative for bacteria, fungi, and mycoplasma detection