HyCyte Cell Line:HyCyte TM3 [Mouse testis] KO-mPnpla6

Parental: TM3 [Mouse testis]

Catalogue No.: CGKO-M3395

Species:Mouse

Gene: mPnpla6

Gene ID: 50767

Passage: 9

Doubling time: 18-24h

Growth Properties: Adherent

Clone Type: Homozygote

Recommended culture media:

Dulbecco's Modified Eagle Medium ,High Glucose(DMEM) supplemented with 10 % FBS, 100 U/mL Penicilin and 100 µg/mL Streptomycin (HyCyte,TCH-G453).

Subculture: Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO₂. Cells should be passaged every 2-3 days. Split at 80-85% confluency, approximately 1:3-1:6.

Freezing media: DMEM + 30 % FBS + 10 % DMSO;or One Step Freezing Medium (HyCyte, GUCP-R201).

Genotype: mPnpla6 (-/-)

Quality test: Negative for bacteria, fungi, and mycoplasma detection