HyCyte Cell Line: HyCyte THLE-2 KO-hTWIST2

Parental: THLE-2

Catalogue No.: CGKO-M3406

Species: Human

Gene:hTWIST2

Gene ID: 117581

Passage: 9

Doubling time: 24-36h

Growth Properties: Adherent

Clone Type: Homozygote

Recommended culture media:

THIE-2 Basal Medium supplemented with 10% FBS, 1% Growth Factor Supplement, and 100 U/mL Penicilin and 100 µg/mL Streptomycin (HyCyte,TCH-G620).

Subculture:Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO2. Cells should be passaged every 2-3 days. Split at 80-85% confluency, approximately 1:2-1:4.

Freezing media:DMEM + 30 % FBS + 10 % DMSO;or One Step Freezing Medium (HyCyte, GUCP-R201).

Genotype:hTWIST2 (-/-)

Quality test:

Negative for bacteria, fungi, and mycoplasma detection