HyCyte Cell Line: HyCyte UM-UC-3 KO-hPRDM1

Parental: UM-UC-3

Catalogue No.: CGKO-M3443

Species: Homo sapiens, human

Gene: hPRDM1

Gene ID: 639

Passage: 9

Doubling time: 18--24h

Growth Properties: Adherent

Recommended culture media:

Minimum Essential Medium (MEM) supplemented with 10 % FBS, 100 U/mL Penicilin and 100 µg/mL Streptomycin (HyCyte,TCH-G369).

Subculture: Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO2. Cells should be passaged every 2-3 days. Split at 80-85% confluency, approximately 1:3-1:6.

Freezing media: MEM + 30 % FBS + 10 % DMSO;or One Step Freezing Medium (HyCyte, GUCP-R201).

Genotype: hPRDM1 (-/-)