HyCyte Cell Line: HyCyte U2OS KO-hSOX9

Parental: U2OS

Catalogue No.: CGKO-M3507

Species: Homo sapiens, human

Gene: hSOX9

Gene ID: 6662

Passage: 9

Doubling time: 18--24h

Growth Properties: Adherent

Clone Type: Homozygote

Recommended culture media:

McCoy's 5A Medium supplemented with 10 % FBS, 100 U/ml Penicilin and 100 µg/ml Streptomycin (HyCyte,TCH-G365).

Subculture: Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO₂. Cells should be passaged every 2-3 days. Split at 80-85% confluency, approximately 1:3-1:6.

Freezing media: McCoy's 5A + 30 % FBS + 10 % DMSO;or One Step Freezing Medium (HyCyte, GUCP-R201)

Genotype: Adherent

Quality test: hSOX9 (-/-)