HyCyte cell line: HyCyte HEI-OC1 mAtp6v1c1_p.R281P_c.842G＞C cell line

Catalogue No.:CGMU-M2290

Parental:HEI-OC1

Gene: mAtp6v1c1

Gene ID: 66335

Genotype：Homozygous

Mutation：p.R281P_c.842G＞C

Species：mouse

Passage: 9

Doubling time: 24--48h

Growth Properties：Adherent

Recommended culture media:

Dulbecco's Modified Eagle Medium ,High Glucose(DMEM) supplemented with 10 % FBS, 100 U/mL Penicilin and 100 µg/mL Streptomycin (HyCyte,TCH-G616).

Subculture:Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO2. Cells should be passaged every 2-3 days. Split at 80-85% confluency, approximately 1:3-1:6.

Freezing media: DMEM + 30 % FBS + 10 % DMSO;or One Step Freezing Medium (HyCyte, GUCP-R201).