HyCyte cell line: HyCyte NK92-MI NF-κB-Luc Overexpression Stable Cell Line

Catalogue No.: CGSC-M2092

Gene: NFkb-Luc

Gene ID: ——

Transduction/transfection methods: Lentivirus

Resistance concentration (μg/mL)：1.5

Target transcript: NM_000566.4

Modification type: Single Clone

Fluorescent gene: Luc&mCherry

Resistance gene: Puro

Parental：NK-92MI

Species：Human

Passage：5

Doubling time：48-72 h

Growth Properties: Suspension

Recommended culture media: Minimum Essential Medium α(MEM α)supplemented with 12.5 % FBS, 12.5 % HS, 0.2 mM Inositol, 0.05 mM β-mercaptoethanol, 0.02 mM Folic Acid, 100 U/mL Penicillin and 100 µg/mL Streptomycin (HyCyte, TCH-G293).

Subculture: Cells are cultured in suspension at 37 °C in a humidified atmosphere with 5% CO₂ and passaged every 2–3 days at a density of 2–3×10⁶ cells/mL, with a seeding density of 5×10⁵ to 1×10⁶ cells/mL.

Freezing media: RPMI-1640 + 30 % FBS + 10 % DMSO; or One Step Freezing Medium (HyCyte, GUCP-R201).