HyCyte cell line: HyCyte 4T1 stable cell line for hCD274 overexpression

Catalogue No.: CGSC-M26861

Gene: hCD274

Gene ID: 29126

Transduction/transfection methods: Transposon

Resistance concentration (μg/mL)：4

Target transcript: NM_014143.4

Modification type: Poolcells

Fluorescent gene: ——

Resistance gene: Puro

Parental：4T1

Species：Mouse

Passage：5

Doubling time：18-24h

Growth properties: Adherent

Recommended culture media: RPMI-1640 supplemented with 10 % FBS, 100 U/mL Penicilin and 100 µg/mL Streptomycin (HyCyte,TCM-G705).

Subculture: Cells are cultured as a monolayer at 37℃ in a humidified atmosphere with 5% CO₂. Cells should bepassaged every 2-3 days. Split at 80-85% confluency, approximately 1:3-1:6.

Freezing media: RPMI-1640 + 30 % FBS + 10 % DMSO;or One Step Freezing Medium(HyCyte, GUCP-R201).