HyCyte cell line: HyCyte 786-O stable cell line for hPOU5F1 overexpression

Catalogue No.: CGSC-M28941

Gene: hPOU5F1

Gene ID: 5460

Transduction/transfection methods: Transposon

Resistance concentration (μg/mL)：2

Target transcript: NM_002701.6

Modification type: Poolcells

Fluorescent gene: EGFP

Resistance gene: Puro

Parental：786-O

Species：Human

Passage：5

Doubling time：48-60h

Growth properties: Adherent

Recommended culture media: RPMI-1640 supplemented with 10 % FBS, 100 U/mL Penicilin and 100 µg/mL Streptomycin(HyCyte,TCH-G107).

Subculture: Cells are culturedas a monolayer at 37℃ in a humidified atmosphere with 5% CO₂. Cells should bepassaged every 2-3 days. Split at 80-85% confluency, approximately 1:3-1:4.

Freezing media: RPMI-1640 + 30 % FBS + 10 % DMSO;or One Step Freezing Medium(HyCyte, GUCP-R201).