HyCyte cell line: HyCyte A20 stable cell line for mItgb1 overexpression

Catalogue No.: CGSC-M32161

Gene: mItgb1

Gene ID: 16412

Transduction/transfection methods: Transposon

Resistance concentration (μg/mL)：2

Target transcript: ——

ModificationType: Poolcells

Fluorescent gene: EGFP

Resistance gene: Puro

Parental：A20

Species：Mouse

Passage：5

Doubling time：18--24h

Growth properties: Suspension

Recommended culture media:

RPMI-1640 supplemented with 10 % FBS,0.05 mM β-mercaptoethanol, 100 U/ml Penicilin and 100 µg/ml Streptomycin (HyCyte,TCM-G804).

Subculture: Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO₂. Cells should be passaged every 2-3 days. The cell culture density is 3×10⁵ to 5×10⁵ cells per mL.

Freezing media: RPMI-1640 + 30 % FBS + 10 % DMSO;or One Step Freezing Medium(HyCyte, GUCP-R201).

Genotype: mCalr (-/-)

Quality test: Bacteria, fungi and mycoplasma negative