HyCyte cell line: HyCyte 3T3-L1 stable cell line for mGpx4 overexpression

Catalogue No.: CGSC-M33351

Gene: mGpx4

Gene ID: 625249

Transduction/transfection methods: Transposon

Resistance concentration (μg/mL)：4

Target transcript: NM_001037741.4

Modification type: Poolcells

Fluorescent gene: — —

Resistance gene: Puro

Parental：3T3-L1

Species：Mouse

Passage：5

Doubling time：18--24h

Growth properties: Suspension

Recommended culture media: Dulbecco's Modified Eagle Medium ,High Glucose(DMEM) supplemented with 10 % CS, 100 U/mL Penicilin and 100 µg/mL Streptomycin (HyCyte,TCM-G702).

Subculture: Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO2. Cells should be passaged every 2-3 days. Split at 80-85% confluency, approximately 1:3-1:4.

Freezing media: DMEM + 30 % FBS + 10 % DMSO;or One Step Freezing Medium (HyCyte, GUCP-R201).