Product Name：Adipogenic Differentiation Medium for Mesenchymal Stem Cell

Catalog No.： GUMD-D102

Size：200 mL

Product Introduction

Under the induction of culture medium, mesenchymal stem cells (MSCs) gradually differentiate into preadipocytes and adipocytes, forming lipid droplets of varying sizes. Oil Red O has a higher solubility in fat than in the staining solution, causing the fat to be stained red or orange-red. This product is suitable for adipogenic induction of MSCs and can significantly improve induction efficiency.

Product Features

· Stable performance, significantly enhancing the adipogenic induction efficiency of MSCs.

· Includes staining solution for convenient use.

· Ready-to-use product, saving time and effort.

NOTE: The adipogenic differentiation level of mesenchymal stem cells varies depending on cell type, donor source, culture conditions, passage number, cell state, differentiation duration, and other factors.

Example: Adipogenic Induction Steps

(The following steps are for reference only. For details, refer to the product manual.)

1. Seeding MSCs: Take logarithmically growing cells and seed them into coated culture vessels at a density of 2×10^4 cells/cm². Culture at 37°C, 5% CO₂ until 90-100% confluency is reached. Discard the supernatant and add adipogenic induction differentiation medium.

2.Cell Differentiation Induction: Culture at 37°C, 5% CO₂ for approximately 3 days, then replace with adipogenic induction differentiation maintenance medium. After 1 day, switch back to the induction medium and culture for another 3 days.

Repeat this medium replacement cycle for 14-21 days, monitoring morphological changes. Determine the termination time of induction based on the number and size of lipid droplets formed, followed by staining for evaluation.

3.Cell Fixation: Remove the medium and wash once with 1× PBS. Cover the bottom of the culture vessel with 4% neutral formaldehyde solution and fix at room temperature for 30-60 min. Discard the fixative and wash twice with 1× PBS.

4.Oil Red O Staining: Prepare Oil Red O working solution by mixing the stock solution with saline or 1× PBS (Oil Red O stock solution : saline = 3:2). Prepare fresh before use.

Add an appropriate amount of working solution to the washed induction wells and incubate for 30 min. Remove the staining solution, wash twice with 1× PBS, and add 1× PBS to prevent drying.

5.Induction Evaluation: Observe adipogenic staining under a microscope, capture images, and evaluate induction efficiency. Successful induction is indicated by lipid droplets stained red or orange-red due to Oil Red O binding.