HyCyte Cell Line: HyCyte NCI-H460 KO-hIMPDH2

Parental: NCI-H460

Catalogue No.: CGKO-M3459

Species: Homo sapiens, human

Gene: hTRIM28

Gene ID: 10155

Passage: 9

Doubling time: 36--48h

Growth Properties: Adherent

Clone Type: Homozygote

Recommended culture media:

RPMI-1640 supplemented with 10 % FBS, 100 U/ml Penicilin and 100 µg/ml Streptomycin (HyCyte,TCH-G286).

Subculture: Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO₂. Cells should be passaged every 2-3 days. Split at 80-85% confluency, approximately 1:3-1:6.

Freezing media: RPMI-1640 + 30 % FBS + 10 % DMSO;or One Step Freezing Medium(HyCyte, GUCP-R201).

Genotype: hTRIM28 (-/-)

Quality test: Bacteria, fungi and mycoplasma negative