Product Name：Luciferase Reporter A-431 Cell Line

HyCyte cell line：HyCyte A-431-Luc

Catalog No.：TCH-C115L

Construction method：PiggyBac

Selection Marker：Puromycin

Parental：A-431

Species：Human

Morphology：Epithelioid

Growth properties：adherent

Culture Medium： DMEM-H+10%FBS+1% Glutamax+1% Sodium Pyruvate+2.0 ug/mL Puromycin+1%P/S

Passage： 1:3-1:6

Doubling time：36-48 h

Subculture：Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO₂. Cells should be passaged every 2-3 days. Split at 80-85% confluency, approximately 1:3-1:4.

Freezing media：DMEM+ 30 % FBS + 10 % DMSO;or One Step Freezing Medium (HyCyte, GUCP-R201).