Product Name：Luciferase Reporter 4T1.2 Cell Line

HyCyte cell line：HyCyte 4T1.2-Luc

Catalog No.：TCM-C810L

Construction method：PiggyBac

Selection Marker：Puromycin

Parental：4T1.2

Species：Mouse

Morphology：Epithelioid

Growth properties：Adherent

Culture Medium : RPMI-1640＋10% FBS+4.0 ug/mL Puromycin＋1% P/S

Passage ratio：RPMI-1640 supplemented with 10 % FBS,4.0 ug/mL Puromycin, 100 U/mL Penicilin and 100 µg/mL Streptomycin (HyCyte,TCM-G810L).

Passage : 1:2-1:4

Doubling time：24-48 h

Subculture：Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO₂. Cells should be passaged every 2-3 days. Split at 80-85% confluency, approximately 1:2-1:4.

Freezing media：RPMI-1640+ 30 % FBS + 10 % DMSO;or One Step Freezing Medium (HyCyte, GUCP-R201).