The COS-7 cell line is derived from CV-1 cells and was obtained by transfection with SV40 carrying an initiation-inactivating mutation encoding the wild-type T antigen. COS-7 cells contain a constitutive copy of the entire early region of the SV40 genome.

Cell Name: African green monkey kidney fibroblast-like cell line

Cell Synonyms: Cos-7; COS7; Cos7; CV-1 in Origin Simian-7

Catalogue No.: TCO-C016
Species: Monkey
Tissue Origin: Kidney
Disease Characteristics: —
Morphology: Fibroblast-like

Growth Properties: Adherent

Culture Medium: DMEM-H + 10% FBS+ 1% P/S
Matching Medium Catalog Number: TCO-G016

Passage Ratio: 1:2-1:4, with medium renewal every 2-3 days
Doubling Time: 24-48 h
Culture Conditions: Atmosphere: 95% air + 5% CO₂, Temperature: 37°C

Freezing Conditions: 60% basal medium + 30% FBS + 10% DMSO, stored in liquid nitrogen

Recommended HyCyte® One-Step Cryopreservation Medium (ready-to-use, serum-free, no programmed cooling required), Product No.: GUCP-R201
Quality Control: Negative for bacteria, fungi, and mycoplasma detection