CV-1 [Part of the Wistar Special Collection] cells are derived from the kidney of an adult male African green monkey; CV-1 [Part of the Wistar Special Collection] cells are commonly used in transfection studies of Rous sarcoma virus.

Cell Name: African green monkey kidney fibroblast cell line

Cell Synonyms: CV-1; Cv-1; CV 1; CV-1.K; CV1

Catalogue No.: TCO-C017
Species: Monkey
Tissue Origin: Kidney
Disease Characteristics: —
Morphology: Fibroblast-like

Growth Properties: Adherent

Culture Medium: DMEM-H + 10% FBS+ 1% P/S
Matching Medium Catalog Number: TCO-G017

Passage Ratio: 1:2-1:3, with medium renewal every 2-3 days
Doubling Time: 24-48 h
Culture Conditions: Atmosphere: 95% air + 5% CO₂, Temperature: 37°C

FreezingConditions: 60% basal medium + 30% FBS + 10% DMSO, stored in liquid nitrogen

Recommended HyCyte® One-Step Cryopreservation Medium (ready-to-use, serum-free, no programmed cooling required), Product No.: GUCP-R201
Quality Control: Negative for bacteria, fungi, and mycoplasma detection