Cell Name: Bovine mammary epithelial cell line

Cell Synonyms: Mac-T; MacT; MAC-T3; Mammary Alveolar Cells-large T antigen

Catalogue No.: TCO-C040

Species: Bovine

Tissue Origin: Mammary gland
Disease Characteristics: —

Morphology: Epithelial-like
Growth Properties: Adherent

Culture Medium: DMEM-H + 10% FBS+ 1% P/S
Matching Medium Catalog Number: TCO-G040

Passage Ratio: 1:4-1:8, with medium renewal every 2-3 days
Doubling Time: 24-36 h
Culture Conditions: Atmosphere: 95% air + 5% CO₂, Temperature: 37°C

Freezing Conditions: 60% basal medium + 30% FBS + 10% DMSO, stored in liquid nitrogen

Recommended HyCyte® One-Step Cryopreservation Medium (ready-to-use, serum-free, no programmed cooling required), Product No.: GUCP-R201
Quality Control: Negative for bacteria, fungi, and mycoplasma detection