Product Name：One-step Freezing Medium for PBMCs

Catalog No.： GUCP-R203

Specification：50 mL

HyCyte®   One-Step Freezing Medium for PBMCs(Serum-Free) is a cryopreservation solution developed by Hyysigen to facilitate the rapid cryopreservation of Peripheral Blood Mononuclear Cells (PBMCs). This medium is specifically designed to accommodate the characteristics of immune cells and NK cells found in PBMC. It minimizes damage to NK cells during cryopreservation, preserves the original proportions of various cell types, and maintains a high recovery rate of NK cells, ensuring successful cryopreservation and subsequent recovery of PBMCs.

The product is user-friendly and does not require any special equipment or instruments, nor does it necessitate a controlled-rate freezing procedure. After resuspending the cells, they can be directly placed in a -80°C freezer to complete the cryopreservation process. All components of this solution are of high purity and possess stable chemical properties, utilizing pharmaceutical excipients. Additionally, the formula is protein-free, which helps prevent protein contamination, making it suitable for applications involving immune cells and NK cells.

Cryopreservation Steps for HyCyte® One-Step Freezing Medium for PBMCs:

1. Collect PBMCs (Peripheral Blood Mononuclear Cells) or cells from the buffy coat layer into a centrifuge tube.

2. Calculate the required number of cells for cryopreservation based on the cell density in culture and the size of the cryovials to be used. The reference quantity is 5×10^5 to 1×10^7 cells/mL.

3. Centrifuge the cells to collect them, typically at 4°C.

4. Carefully collect the cell pellet and completely discard the supernatant from the centrifuge tube.

5. Add an appropriate amount of HyCyte® PBMC-Specific One-Step Cryopreservation Solution, pre-cooled to 4°C, into the centrifuge tube. Gently mix the cells to create a cell suspension.

6. Aliquot the cell suspension from the centrifuge tube into labeled cryovials or cryobags. It is recommended to fill each vial with 1 mL or 1.5 mL of suspension or follow the instructions provided for cryobags.

7. Immediately place the cryovials in a -80°C freezer for rapid cryopreservation. After 24 hours, transfer them to liquid nitrogen for long-term storage or keep them at -80°C.

Note:   Ensure that the cryovials or bags are not in close contact with each other during freezing. They should be spaced apart to avoid interference with the cooling process of the cells.