HyCyte Cell Line: HyCyte NCI-H1703 KO-hGALNT14

Parental: NCI-H1703

Catalogue No.: CGKO-M2991

Species: Human

Gene: hGALNT14

Gene ID: 79623

Passage: 9

Doubling time: 24-48 h

Growth Properties: Adherent

Clone Type: Homozygote

Recommended culture media:

RPMI-1640 supplemented with 10 % FBS, 2mM Glutamax,1mM Sodium Pyruvate,100 U/mL Penicilin and 100 µg/mL Streptomycin (HyCyte,TCH-G436).

Subculture: Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO₂. Cells should be passaged every 2-3 days. Split at 80-85% confluency, approximately 1:3-1:4.

Freezing media: RPMI-1640 + 30 % FBS + 10 % DMSO;or One Step Freezing Medium(HyCyte, GUCP-R201).

Genotype: hGALNT14 (-/-)

Quality test: Bacteria, fungi and mycoplasma negative