HyCyte Cell Line: HyCyte HGC-27 KO-hERV3-1

Parental: HGC-27

Catalogue No.: CGKO-M3365

Species: Human

Gene: hERV3-1

Gene ID:2086

Passage: 9

Doubling time: 18-24h

Growth Properties: Adherent

Clone Type: Homozygote

Recommended culture media:

RPMI-1640   supplemented with 10 % FBS, 100 U/ml Penicilin and 100 µg/ml

Streptomycin (HyCyte,TCH-G202）

Subculture: Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO₂. Cells should be passaged every 2-3 days. Split at 80-85% confluency, approximately 1:3-1:6

Freezing media: RPMI-1640 + 30 % FBS + 10 % DMSO;or One Step Freezing Medium(HyCyte, GUCP-R201)

Genotype: hERV3-1 (-/-)

Quality test: Bacteria, fungi and mycoplasma negative