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发货周期：
干冰冻存管运输3天（1x106cells/冻存管x2）
常温运温1-2周发货 (1x106 cells/T25培养瓶）
原价：1500元 套装价特惠价：890元
1948年三月建立了NCTCclone929(小鼠结缔组织)，细胞系L的克隆。细胞系L是较早建立的连续培养细胞系之一，而clone929是较早的克隆株。从一只100日龄的雄性C3H/An小鼠的正常皮下疏松结缔组织入脂肪组织中建立了亲本细胞系L。第95代的细胞系L使用毛细管法分离单细胞建立了clone929。检测发现鼠痘病毒阴性。
细胞名称：小鼠成纤维细胞
细胞简称：L-929
产品货号：TCM-C749
种属来源：小鼠
组织来源：皮下结缔组织
疾病特征：/
细胞形态：成纤维细胞样
生长特性：贴壁生长
培养体系：MEM+10%马血清+1%P/S
配套培养基货号：TCM-G749
传代比例：1:3-1:6，每2-3天换液一次
倍增时间：48-72 h
培养条件：气相：95%空气+5%CO2，温度：37℃
冻存条件：60%基础培养基+30%FBS+10%DMSO，液氮储存
质量检测：细菌、真菌、支原体检测均为阴性
参考文献：
"Kazazian HH Jr., et al. Restriction site polymorphism in the phosphoglycerate kinase gene on the X chromosome. Hum. Genet. 66: 217-219, 1984. PubMed: 6325324
Fisher EM, et al. Homologous ribosomal protein genes on the human X and Y chromosomes: escape from X inactivation and possible implications for Turner syndrome. Cell 63: 1205-1218, 1990. PubMed: 2124517
Sanford KK, et al. The growth in vitro of single isolated tissue cells. J. Natl. Cancer Inst. 9: 229-246, 1948.
Sugarman BJ, et al. Recombinant human tumor necrosis factor-alpha: effects on proliferation of normal and transformed cells in vitro. Science 230: 943-945, 1985. PubMed: 393311
ASTM International Standard Practice for Direct Contact Cell Culture Evaluation of Materials for Medical Devices. West Conshohocken, PA:ASTM International;ASTM Standard Test Method F 0813-07.
ASTM International Standard Test Method for Agar Diffusion Cell Culture Screening for Cytotoxicity. West Conshohocken, PA:ASTM International;ASTM Standard Test Method F 0895-84 (Reapproved 2006).
U.S. Pharmacopeia General Chapters:<87>Biological Reactivity Tests, in vitro. Rockville, MD: U.S. Pharmacopeia; USP USP34-NF29, 2011
Westfall BB, et al. The glycogen content of cell suspensions prepared from massive tissue culture: comparison of cells derived from mouse connective tissue and mouse liver. J. Natl. Cancer Inst. 14: 655-664, 1953. PubMed: 13233820
Earle WR, et al. Production of malignancy in vitro. IV. The mouse fibroblast cultures and changes seen in the living cells. J. Natl. Cancer Inst. 4: 165-212, 1943."
L-929 已发表过的文章：
IF=16 Lu Q, Ye C, Mao W, Zeng F, Liu Z, Chen R, Cheng X, Gao Y, Wang M, Liu M, Tang S, Song Y. Targeting Senescent Alveolar Type 2 Cells with a Gene-Editable FePt Dual-Atom Catalyst for Mitigating Idiopathic Pulmonary Fibrosis. ACS Nano. 2025 Jul 1;19(25):23162-23176.
IF=15.7 Jin H, Zhu X, Zhang M, Zheng H, Chen Y, Yao D, Liu S, Zhang H. Cu/Zn/histidine supramolecular assemblies with optimized Cu catalytic sites as an alternative of superoxide dismutase. Nat Commun. 2025 Nov 18;16(1):10075.
IF=11.2 Teng J, Zhao W, Zhang S, et al. Injectable nanoparticle-crosslinked xyloglucan/ε-poly-l-lysine composite hydrogel with hemostatic, antimicrobial, and angiogenic properties for infected wound healing[J]. Carbohydrate Polymers, 2024: 122102.
IF=5.2 Fang Z, Liu C, Cheng Y, Ji Y, Liu C. Combined analysis of bulk, single-cell RNA sequencing, and spatial transcriptomics reveals the expression patterns of lipid metabolism and ferroptosis in the immune microenvironment of metabolic-associated fatty liver disease. Life Sci. 2025 Feb 1;362:123377.
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